Co-reporter:Yanmei Li, Yanfang Wang, Jingjie Ye, Jiang Yuan, Yinghong Xiao
Materials Science and Engineering: C 2016 Volume 68() pp:177-183
Publication Date(Web):1 November 2016
DOI:10.1016/j.msec.2016.05.117
•Keratins are coelectrospun with PCL to afford nanofibrous mats.•PCL/keratin mats show good cytocomatibility and blood compatibility.•PCL/keratin mats have great potential as scaffold for vascular tissue engineering.The natural abundance of cell adhesion sequences, RGD (Arg-Gly-Asp) and LDV (Leu-Asp-Val) in the keratins make them suitable as biomaterials for tissue engineering applications. Herein, keratins were coelectrospun with poly(ε-caprolactone)(PCL) at the ratio of 9/1, 8/2, and 7/3 to afford nanofibrous mats. The resulting mats were surface-characterized by ATR-FTIR, SEM, WCA, and XPS. Cell attachment data showed that NIH 3T3 cells adhered more to the PCL/keratin nanofibrous mats than the pristine PCL mats. The MTT assay revealed that the PCL/keratin mats had improved cell viability. The blood clotting time test (APTT, PT, and TT) indicated the PCL/keratin mats exerted good blood compatibility. These mats would be a good candidate as a scaffold for vascular tissue engineering.
Co-reporter:Jingting Tan, Na Meng, Yunting Fan, Yutian Su, Ming Zhang, Yinghong Xiao, Ninglin Zhou
Materials Science and Engineering: C 2016 Volume 61() pp:681-687
Publication Date(Web):1 April 2016
DOI:10.1016/j.msec.2015.12.098
•Hydroxypropyl-β-cyclodextrin (HP-β-CD) modified carboxylated graphene oxide (GO-COOH) was designed as a drug carrier.•The prepared PTX-loaded nanospheres can be dispersed in aqueous medium stably.•The GO-COO-HP-β-CD nanospheres are safe for blood-contact applications.•This newly developed PTX-delivery system could confer significantly improved cytotoxicity against tumor cells.A novel drug carrier based on hydroxypropyl-β-cyclodextrin (HP-β-CD) modified carboxylated graphene oxide (GO-COOH) was designed to incorporate anti-cancer drug paclitaxel (PTX). The formulated nanomedicines were characterized by Fourier transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). Results showed that PTX can be incorporated into GO-COO-HP-β-CD nanospheres successfully, with an average diameter of about 100 nm. The solubility and stability of PTX-loaded GO-COO-HP-β-CD nanospheres in aqueous media were greatly enhanced compared with the untreated PTX. The results of hemolysis test demonstrated that the drug-loaded nanospheres were qualified with good blood compatibility for intravenous use. In vitro anti-tumor activity was measured and results demonstrated that the incorporation of PTX into the newly developed GO-COO-HP-β-CD carrier could confer significantly improved cytotoxicity to the nanosystem against tumor cells than single application of PTX. GO-COO-HP-β-CD nanospheres may represent a promising formulation platform for a broad range of therapeutic agent, especially those with poor solubility.
Co-reporter:Xue Chen, Xiaoshu Zhu, Yinghong Xiao, Xiaodi Yang
Journal of Electroanalytical Chemistry 2015 Volume 743() pp:99-104
Publication Date(Web):15 April 2015
DOI:10.1016/j.jelechem.2015.02.004
•PEDOT and g-C3N4 were used to modify electrodes by a layer-by-layer assembly method for supercapacitors.•The specific capacitance of PEDOT/g-C3N4 electrode is almost double that of the PEDOT electrode.•Composite electrode represented 17.5 W h kg−1 of E at a P of 5000 W kg−1 in 1 M Na2SO4.•PEDOT/g-C3N4 electrode exhibited over 96.5% capacitance stability after 1000 cycles in 1 M Na2SO4.A novel electrode material for supercapacitor composed of poly(3,4-ethylenedioxythiophene):poly(styrenesulfonate) (PEDOT) and graphitic-phase C3N4 (g-C3N4) was prepared by a layer-by-layer assembly method. The resulting composite of PEDOT/g-C3N4 represented excellent electrochemical stability in neutral electrolyte and enhanced electrochemical performance of capacitance and average energy density in comparison with single PEDOT. The specific capacitance was 137 F g−1 in H2SO4 and 200 F g−1 in Na2SO4 at a current density of 2 A g−1, respectively. More significantly, the average energy density was 9.65 W h kg−1 at an average power density of 4001.86 W kg−1 in H2SO4. While in Na2SO4, the average energy density reached 17.5 W h kg−1 at an average power density of 5000 W kg−1. More than 89% and 96.5% of capacitance were retained in H2SO4 and Na2SO4 respectively, suggesting that PEDOT/g-C3N4 possessed an excellent cycling stability in view of capacity performance as electrode materials. The property improvement was attributed to the synergistic effects of the two components in the composite.
Co-reporter:Ling Gao, Yinghong Xiao, Yanping Wang, Xue Chen, Bo Zhou, Xiaodi Yang
Talanta 2015 Volume 132() pp:215-221
Publication Date(Web):15 January 2015
DOI:10.1016/j.talanta.2014.09.010
•A nanocomposite-based biosensor was constructed by grafting an aptamer onto COO-GR.•COO-GR is advantageous in simplifying sensor preparation.•SWV was employed to realize selective and specific hemin detection.A unique nanocomposite was crafted by grafting hemin-binding-aptamer (HBA) onto carboxylated graphene (COO-GR). Infrared spectroscopy, Raman spectroscopy and diffuse reflectance spectra suggested that –NHCO– covalent bonds were formed between HBA and COO-GR. The resulting COO-GR/HBA functionalized electrode was used as a novel label-free biosensor. The square wave voltammetry was employed to realize the selective and specific detection of hemin. The obtained aptasensor possessed excellent performance with a detection limit of 0.64 nmol L−1 (S/N=3) and a linear range from 1 to 150 nmol L−1. Moreover, COO-GR was shown to be a promising candidate in making aptasensors, carrying advantages over graphene in terms of the simplicity of sensor preparation and the reduction of background noise.An aptasensor constructed by grafting hemin-binding-aptamer (HBA) onto carboxylated graphene (COO-GR) was used to selectively and specifically detect hemin. With the advantage over graphene in simplifying sensor preparation and reducing background noise, the resulting aptasensor possesses excellent performance with a detection limit of 0.64 nmol L−1 (S/N=3) and a linear range from 1 to 150 nmol L−1.
Co-reporter:Hengyang Xiao, Min Zhang, Yinghong Xiao, Jianfei Che
Colloids and Surfaces B: Biointerfaces 2015 Volume 126() pp:138-145
Publication Date(Web):1 February 2015
DOI:10.1016/j.colsurfb.2014.12.004
•Electrophoresis is first introduced to fabricate SWNT/PPy composite film for neural interface.•The pore size of SWNT film can be controlled by adjusting the concentration of Al3+ ions in the electrolyte.•SWNT film fabricated in the study has strong adhesive force.Single walled carbon nanotube (SWNT)/polypyrrole (PPy) composite films with controlled pore size and strong adhesive force was prepared as electrode material for improving the performance of neural electrodes. SWNT film with controlled pore size was first fabricated through electrophoresis with a merit that the pore size can be well tuned by changing the concentration of metal ions in the electrolyte. An ultrathin conformal PPy layer around SWNT bundles in a uniform manner within the entire films was subsequently obtained by pulsed electropolymerization. The adhesion of the SWNT coated electrodes was tested by repeatedly inserting the coated electrode into agar gel to demonstrate the better adhesive force of the coating. Electrochemical results showed that the SWNT/PPy coated metal electrodes have much lower impedance and higher charge storage capacity than the bare metal substrates. Further in vitro culture of rat pheochromocytoma (PC12) cells revealed that the porous SWNT/PPy composite film was non-toxic and well supported the growth of neurons. We demonstrate that the prepared composite film has potential applications in chronic implantable neural electrodes for neural stimulation and recording.
Co-reporter:Nuona A, Xianye Li, Xiaoshu Zhu, Yinghong Xiao, Jianfei Che
Composites Part A: Applied Science and Manufacturing 2015 Volume 69() pp:247-254
Publication Date(Web):February 2015
DOI:10.1016/j.compositesa.2014.11.025
The high production cost of polylactide (PLA) can be effectively reduced by simply mixing with starch, unfortunately a trade-off of its mechanical properties. In this paper, we reported a new strategy in which graphene oxide (GO) was used as a compatibilizer to bridge PLA and starch. The native starch was first cationized and then encapsulated with GO by electrostatic force between the negatively charged GO and the positively charged cationic starch. The encapsulating GO was reduced by the quaternary ammonium ions on the cationic starch, which converted the surface of the starch from hydrophilic to hydrophobic. Due to the amphipathicity approximation between PLA and starch, a good dispersion as well as a strong interfacial adhesion was achieved. The PLA composite reinforced with GO encapsulated starch exhibited much higher yield strength than that of pure PLA, increasing from 36.64 MPa up to 41.40 MPa.
Co-reporter:Xianye Li;Anne Bergeret;Marc Longerey;Jianfei Che
Polymer Composites 2014 Volume 35( Issue 2) pp:396-403
Publication Date(Web):
DOI:10.1002/pc.22673
Polylactide (PLA)/graphene nanocomposites were prepared by a facile and low-cost method of solution-blending of PLA with liquid-phase exfoliated graphene using chloroform as a mutual solvent. Transmission electron microscopy (TEM) was used to observe the structure and morphology of the exfoliated graphene. The dispersion of graphene in PLA matrix was examined by scanning electron microscope, X-ray diffraction, and TEM. FTIR spectrum and the relatively low ID/IG ratio in Raman spectroscopy indicate that the structure of graphene sheets (GSs) is intact and can act as good reinforcement fillers in PLA matrix. Thermogravimetric analysis and dynamic mechanical analysis reveal that the addition of GSs greatly improves the thermal stability of PLA/GSs nanocomposites. Moreover, tensile strength of PLA/GSs nanocomposites is much higher than that of PLA homopolymer, increasing from 36.64 (pure PLA) up to 51.14 MPa (PLA/GSs-1.0). POLYM. COMPOS., 35:396–403, 2014. © 2013 Society of Plastics Engineers
Co-reporter:Min Zhang, Yaochen Ma, Yinyan Zhu, Jianfei Che, Yinghong Xiao
Carbon 2013 Volume 63() pp:149-156
Publication Date(Web):November 2013
DOI:10.1016/j.carbon.2013.06.066
Transparent hydrophobic coatings were prepared with graphene fluoride (GF), which was fabricated by a mild and controllable approach of liquid-phase ultrasonic exfoliation of graphite fluoride (GiF) with a selected solvent of ethanol. The structure of the resulting GF sheets with nanoscale thickness was confirmed by transmission electron microscope (TEM), atomic force microscopy (AFM), X-ray diffraction and Raman spectroscopy. Fourier transform infrared spectroscopy and energy dispersive analysis show that C–F bonds from GiF are well preserved in the resulting GF by the method of liquid-phase ultrasonic exfoliation, which are responsible for its excellent hydrophobicity and light transmission. The obtained GF coating has a contact angle of 123° and light transmittance of up to 92% when GF mass density is 0.6 μg cm−2. Water erosion experiments and ultraviolet aging tests suggest these coatings may possess extended service life outdoors.
Co-reporter:Yinghong Xiao, Yanping Wang, Min Wu, Xiaoling Ma, Xiaodi Yang
Journal of Electroanalytical Chemistry 2013 Volume 702() pp:49-55
Publication Date(Web):1 August 2013
DOI:10.1016/j.jelechem.2013.05.010
•A unique nanocomposite was constructed by grafting an aptamer on graphene.•The functionalized electrode can be used as a biosensor for lysozyme detection.•The novel label-free aptasensor shows a detection limit of 6 fmol L−1.•We investigated the effect of electric feature of the functionalized surface on charge transfer.This work presents a facile route to fabricate a highly sensitive platform for the recognition of lysozyme. Graphene oxide was coated on the surface of a glassy carbon electrode (GCE) followed by reduction with hydrazine to obtain a graphene (GR) coated GCE. After activation of GR–GCE with EDC and NHS, lysozyme binding aptamer (LBA) was molecularly tethered onto GR to obtain a highly sensitive platform named as LBA–GR–GCE. Results show that GR greatly promotes the electron transfer at electrode/electrolyte interface due to its excellent electrical conductivity. LBA–GR–GCE is able to quantitatively detect the analyte of lysozyme in real samples of human saliva and chicken egg white with high selectivity, with a linear range of 0.01–0.5 pmol L−1 and detection limit of 6 fmol L−1 (S/N = 3). Furthermore, the surface feature of the functionalized electrode was investigated to determine its effect on charge transfer resistance with two different electrochemical probes of [Fe(CN)6]3−/4− and Ru(NH3)63+.
Co-reporter:Xiaoying Tong, Cuncun Xie, Ling Si, Jianfei Che, Yinghong Xiao
Materials Chemistry and Physics 2013 Volume 143(Issue 1) pp:85-92
Publication Date(Web):16 December 2013
DOI:10.1016/j.matchemphys.2013.08.017
•Highly stable graphene dispersion was prepared by exfoliation with the aid of Nafion.•Low-temperature annealing was employed to treat graphene films for the first time.•Graphene films possess sheet resistance of 2.86 KΩ sq−1 and light transmittance over 84%.This article presents a novel and simple method of liquid-phase exfoliation to fabricate graphene films that possess high conductivity and good light transparency. Graphite was exfoliated in water–ethanol mixture, with the aid of Nafion, to give highly stable graphene dispersion. Transparent graphene thin films were easily deposited by vacuum filtration from the Nafion-stabilized graphene dispersion. More important, low-temperature air-annealing (at 250 °C for 2 h) was employed to treat freshly-prepared graphene films for the first time. It demonstrates that the technique is advantageous and quite efficient for the fabrication of exfoliated graphite films with defect-free structure and high purity, confirmed by TEM, SEM, FTIR, XPS, and Raman spectra. The resulting graphene films possess a sheet resistance lower than 2.86 kΩ sq−1 and optical transmittance over 84% at a typical wavelength of 550 nm.
Co-reporter:Yinghong Xiao, Lei He and Jianfei Che
Journal of Materials Chemistry A 2012 vol. 22(Issue 16) pp:8076-8082
Publication Date(Web):24 Feb 2012
DOI:10.1039/C2JM30601H
High-quality conductive composite hydrogels composed of single-walled carbon nanotubes (SWNTs), polypyrrole (PPy) and poly(ethylene glycol) diacrylate (PEGDA) hydrogel were successfully fabricated through interfacial polymerization (IP). Compared to the conventional sequential interpenetrating polymerization (CI), IP is superior in improving the electrical/electrochemical properties of the incorporated hydrogel due to the higher content of PPy up to 14.1 wt% in its dry weight. The electrical conductivity of PPy/PEGDA hydrogel is nearly more than two orders of magnitude higher than that of the hydrogel prepared via CI. Furthermore, simultaneous incorporation of SWNTs and PPy results in significantly enhanced compressive modulus, from 183 ± 13 kPa to 1823 ± 201 kPa. Electrochemical measurements clearly indicate that the electron-transfer resistance is significantly decreased from 9320 Ω of native PEGDA hydrogel to 247 Ω of SWNT/PPy/PEGDA composite hydrogel.
Co-reporter:Yanping Wang, Yinghong Xiao, Xiaoling Ma, Na Li and Xiaodi Yang
Chemical Communications 2012 vol. 48(Issue 5) pp:738-740
Publication Date(Web):25 Nov 2011
DOI:10.1039/C1CC15429J
A unique nanocomposite was constructed by grafting an aptamer on graphene and used as a label-free biosensor for thrombin sensing, showing good selectivity, a low detection limit of 0.45 fM and high sensitivity, with a linear response up to 100 fM.
Co-reporter:Yinghong Xiao;Xinxin Ye;Lei He;Jianfei Che
Polymer International 2012 Volume 61( Issue 2) pp:190-196
Publication Date(Web):
DOI:10.1002/pi.3168
Abstract
A novel drug delivery system (DDS) based on a carbon nanotube (CNT)–poly(3,4-ethylenedioxythiophene) (PEDOT) composite was constructed via a layering method. Single-walled CNTs (SWNTs) were immobilized on a gold electrode using a layer-by-layer technique. In particular, cysteamine (Cys) was firstly bonded to the gold surface through the strong SAu association and SWNTs were subsequently linked onto the Cys layer through condensation reaction of NH2 and carboxyl groups by 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide/N-hydroxysuccinimide coupling. X-ray photoelectron spectroscopy and Raman spectroscopy demonstrate that this is a facile route for immobilizing CNTs on gold electrodes. Finally PEDOT was electropolymerized on the SWNT-functionalized electrode to make a SWNT–PEDOT composite, and the modified electrode was applied as a DDS. Dexamethasone, as a model drug, was incorporated into PEDOT in the electropolymerization. Investigations of the electrochemical properties of SWNT–PEDOT demonstrate that SWNTs greatly improve the conductivity and increase the charge capacity of PEDOT. The composite exhibits a petal-like surface structure, 20–30 nm thick and 100–200 nm wide. Compared to a DDS based on pure PEDOT synthesized under the same conditions, SWNT–PEDOT has the merits of higher drug release rate and larger release amount. The average mass release for every five voltammetry cycles increases from 1.4126 to 1.8864 mg cm−2. Copyright © 2011 Society of Chemical Industry
Co-reporter:Yanping Wang, Yinghong Xiao, Xiaoling Ma, Na Li and Xiaodi Yang
Chemical Communications 2012 - vol. 48(Issue 5) pp:NaN740-740
Publication Date(Web):2011/11/25
DOI:10.1039/C1CC15429J
A unique nanocomposite was constructed by grafting an aptamer on graphene and used as a label-free biosensor for thrombin sensing, showing good selectivity, a low detection limit of 0.45 fM and high sensitivity, with a linear response up to 100 fM.
Co-reporter:Yinghong Xiao, Lei He and Jianfei Che
Journal of Materials Chemistry A 2012 - vol. 22(Issue 16) pp:
Publication Date(Web):
DOI:10.1039/C2JM30601H
